biolasers, new ones that utilize incorporated protein fluorophores within microscale optical cavities to produce coherent light, have a wide range of applications in medicine, including identification, diagnosing, and treating many diseases, skin repair, controlling chemical reactions, pattern generation, etc. Therefore, a new approach to form active microcavities for micro biolaser is proposed based on dye rhodamine B (RhB) and bovine serum albumin (BSA) in the paper. The results indicate the successful production of curved-shaped active cavities. The formed biocavities' average diameters and diameter dispersion are 35.221±0.674 μm and 1.9%, respectively. The output emissions of the device are studied under optical pumping by a Diode-Pumped Solid State (DPSS) laser with the Nd: YAG gain medium operated at 532 nm and an incidence angle of 45º. The output emissions are recorded at different angles, and the high output power is observed at an angle of 90° with the pump angle. Furthermore, the design device has different modes, and the lowest linewidth at half maximum (0.35 nm) and the highest quality factor (2313) are observed at an output wavelength of 809.6 nm. Therefore, the produced laser-activated microcavities can be exploited as suitable options in medical and non-medical optical applications.